<aside> 💡 This shows how to view the sequence of a genome in the genome browser JBrowse

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Requirements

• JBrowse (genome browser): https://jbrowse.org/jb2/
• samtools: https://www.htslib.org/download/
• Your genome file as a fasta file (.fasta or .fa)

Steps

1. Create a fasta index of your genome
2. Load the genome (fasta) together with your fasta index file

1. Create a fasta index of your genome

What’s a fasta index (.fai) file?

samtools faidx <fasta file>

2. Load the genome file into JBrowse

→ Click on OPEN SEQUENCE FILE(S)

→ Select the correct files for the fasta and fasta index

→ Click on OPEN TRACK SELECTOR

→ Select Reference sequence…